Bowser has become invulnerable by imprisoning the seven Star Spirits and using the wish granting powers of the mythical Star Rod from Star Haven. Mario must rescue the Star Spirits, who have been sealed in cards.
Mario and Peach go on a vacation to Isle Delfino. But their trip soon gets spoiled after an imposter disguised as Mario paints and pollutes the entire island. Mario must clear his name, and clean up the entire island.